Testing - FirstResponder

Testing

Aerobiology Laboratory Associates, Inc. out of Golden, Colorado conducted environmental testing swab analysis on the FirstResponder® Sterilizer. Aerobiology Laboratory Associates has the following credentials:

CEICC - ACAC Council-certified Environmental Infection Control Consultant
CIEC - ACAC Council-certified Indoor Environmental Consultant
CMC - ACAC Council-certified Microbial Consultant
CAC - Cal-OSHA Certified Asbestos Consultant (#96-1998)
ICC Certified Combination Residential and Commercial Inspector
  • Multiple pathogens tested using same testing profile for hospital screening (Hospital profile SOP 2.3).


Sample Protocol

Step 1

Swab surface

Step 2

Read swab using Luminometer

Step 3

Perform 1 hour ozone sterilization cycle with FirstResponder® Sterilizer

Step 4

Swab same but adjacent area and read again using Luminometer


Results

Test #1

In-house testing using the Bio Reveal system SURE II™ ATP bioluminescence luminometer and Hygiena Ultrasnap™ swabs. As shown in Table 1 below, the highest Luminometer reading was 4,705 RLUs prior to FirstResponder® Sterilizer treatment, yet a reading over 30 is considered “surface contaminated” according to manufacturer.

Post-treatment Luminometer readings were taken after FirstResponder® Sterilizer one hour treatment and readings show a dramatic reduction or elimination of surface contaminant as in Table 1 below (all readings were done in triplicate to insure accuracy):


TABLE 1: Surface contaminant pre and post treatment with FirstResponder® Sterilizer.

Location Pre-treatment * Post-treatment *
Door Handle 14 0
Biohazard Container Top 4,705 18
Gurney Handle 272 0
Ceiling Handrail 17 0
Floor (rear, right entry) 247 0
Bench Seat 109 0
Blood Pressure Cuff 216 5
Driver Steering Wheel 24 0
* Numbers represent relative light units (RLUs) read by luminometer and are directly correlated to the presence of biological materials,



Test #2

Via a certified environmental consulting expert and a third party independent testing laboratory (Aerobiology Laboratory Associates, Inc., Golden, Colorado).

Graph and table 2 below show the elimination or dramatic reduction in species-specific bacterial contaminants. Samples were taken from 12 discreet locations inside an ambulance and immediately sent on ice to testing laboratory.



GRAPH 1: graphic representation of results in TABLE 2 below.




TABLE 2: Contaminant species presence using testing profile for hospital screening (Hospital profile SOP 2.3).performed by third-party testing lab.
BEFORE FirstResponder® AFTER FirstResponder®
Bacillus species 6,100 7
Coag-negative Staphylococcus 800 0
Pseudomonas straminea 195 0
Roseomonas 70 0

Partial List of Pathogens FirstResponder® Sterilizer Kills

Bacteria
  • Achromobacter butyri NCI-9404
  • Aeromonas harveyi NC-2
  • Aeromonas salmonicida NC-1102
  • Bacillus anthracis
  • Bacillus cereus
  • Bacillus coagulans
  • Bacillus globigii
  • Bacillus licheniformis
  • Bacillus megaterium sp.
  • Bacillus paratyphus
  • Bacillus prodigiosus
  • Bacillus subtilis
  • Bacillus stearothermophilus
  • Clostridium botulinum
  • Clostridium Difficile
  • Clostridium sporogenes
  • Clostridium tetani
  • Corynebacterium diphtheriae
  • Eberthella typhosa
  • Escherichia coli K-12
  • Escherichia coli B
  • Flavobacterium SP A-3
  • Leptospira canicola
  • Listeria sp.
  • Micrococcus candidus
  • Micrococcus caseolyticus KM-15
  • Micrococcus sphaeroides
  • MRSA
  • Mycobacterium leprae
  • Mycobacterium tuberculosis
  • Mycoplasma spp.
  • Neisseria catarrhalis
  • Phytomonas tumefaciens
  • Proteus vulgaris
  • Pseudomonas aeruginosa
  • Pseudomonas fluorescens (biofilms)
  • Pseudomonas putida
  • Salmonella choleraesuis
  • Salmonella enteritidis
  • Salmonella typhimurium
  • Salmonella typhosa
  • Salmonella paratyphi
  • Sarcina lutea
  • Serratia marcescens
  • Shigella dysenteriae
  • Shigella flexneri
  • Shigella paradysenteriae
  • Spirillum rubrum
  • Staphylococcus albus
  • Staphylococcus aureus
  • Streptococcus C
  • Streptococcus faecalis
  • Streptococcus hemolyticus
  • Streptococcus lactis
  • Streptococcus salivarius
  • Streptococcus viridans
  • Vibrio alginolyticus
  • Vibrio anguillarum
  • Vibrio cholerae
  • Vibrio comma
  • Vibrio ichthyodermis NC-407
  • Vibrio parahaemolyticus
  • VRE
  • VRSA
Protozoa
  • Cryptosporidium parvum
  • Cyrptosporidium sp.
  • Giardia lamblia
  • Giardia muris
  • Paramecium
  • Nematode eggs
  • All Pathogenic and Non-pathogenic forms of Protozoa
  • Entamoeba histolytica
Fungus
  • Alternaria solani
  • Aspergillus candidus
  • Aspergillus flavus (yellowish-green)
  • Aspergillus glaucus (bluish-green)
  • Aspergillus niger (black)
  • Aspergillus terreus
  • Aspergillus saitoi
  • Aspergillus oryzae
  • Botrytis allii
  • Botrytis cinerea
  • Colletotrichum lagenarium
  • Fusarium oxysporum
  • Geotrichum sp.
  • Monilinia fruiticola
  • Monilinia laxa
  • Mucor racemosus A & B (white-gray)
  • Mucor piriformis
  • Oospora lactis (white)
  • Penicillium cyclopium
  • Penicillium chrysogenum
  • Penicillium citrinum
  • Penicillium digitatum (olive)
  • Penicillium glaucum
  • Penicillium expansum (olive)
  • Penicillium egyptiacum
  • Penicillium roqueforti (green)
  • Pythium ultimum
  • Phytophthora erythroseptica
  • Phytophthora parasitica
  • Rhizoctonia solani
  • Rhizopus nigricans (black)
  • Rhizopus stolonifer
  • Sclerotium rolfsii
  • Sclerotinia sclerotiorum
  • Thamnidium sp.
  • Trichoderma viride
  • Verticillium albo-atrum
  • Verticillium dahliae
Virus
  • Adenovirus (type 7a)
  • Bacteriophage T1 (E.coli)
  • Coxsackie A9, B3, & B5
  • Cryptosporidium
  • Vesicular Stomatitis
  • Echovirus 1, 5, 12, &29
  • Encephalomyocarditis
  • Hepatitis
  • HIV
  • Infectious hepatitis
  • Influenza
  • Legionella pneumophila
  • Poliovirus (Poliomyelitis) 1, 2 & 3
  • Rotavirus
  • Tobacco mosaic
Yeast
  • Baker’s yeast
  • Candida albicans - all forms
  • Common yeast cake
  • Saccharomyces cerevisiae
  • Saccharomyces ellipsoideus
  • Saccharomyces sp.
  • Torula rubra
Algae
  • Chlorella vulgari